Allergenicity & celiac disease - bioinformatics

Why?

One key aspect in the safety assessment of enzymes and other proteins is the potential allergenicity. The assessment is a stepwise process, in which bioinformatic analysis is usually the first screening step.

How?

The DNA sequence from which the protein sequence can be inferred, or the actual protein sequence, will be used for the analysis. Several databases are available, which are built based on different principles. Some of them are very stringent in the inclusions, whereas others include proteins based on protein similarity without any real evidence for allergenicity. Some of the databases include proteins, which can be used for the assessment of the potential contribution to celiac disease.

A routine, internationally accepted approach is to run a sliding window search of 80 amino acids through the protein and look for any window having 35% or higher identity. It is also possible to predict all proteins from the whole genome sequence of a microorganism and make an allergen database search.

Biosafe Ltd uses several databases, compares the results and carefully assesses the outcome.

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